[{"year":"2021","keyword":["3D printing","FDM printing","sterilization","adherent cells","CHO cells"],"intvolume":" 8","main_file_link":[{"open_access":"1"}],"funded_apc":"1","oa":"1","type":"journal_article","quality_controlled":"1","volume":8,"publication":"AIMS Bioengineering","user_id":"223776","issue":"1","date_updated":"2022-01-01T15:23:09Z","article_type":"original","status":"public","abstract":[{"lang":"eng","text":" Cell growth on three-dimensional objects is of high interest for bioprocess engineering of adherent cells, tissue engineering and other biomedical applications. 3D printing by fusion deposition modeling (FDM) is a cheap possibility to generate tailor-made substrates for cell growth. Nevertheless, not all materials are chemically attractive for cells. Polylactic acid (PLA) and polyethylene terephthalate glycol (PETG) have been reported to be suitable polymers for tissue engineering. Thus, they might also be applicable for cost-effective bioprocessing of adherent cell lines. Here we report on the influence of printing material, surface structure, and sterilization method on Chinese hamster ovary (CHO) cell adhesion on a modified, high temperature resistant PLA, a PETG blend, and unmodified PETG, respectively. Our study revealed that CHO cells grew on all polymers tested without further surface modification. Samples could be efficiently chemically sterilized. Additional acid treatment had no significant effect on cell adhesion. "}],"title":" Mammalian cell adhesion on different 3D printed polymers with varying sterilization methods and acidic treatment","date_created":"2021-01-03T18:14:05Z","file_date_updated":"2021-01-03T18:13:31Z","_id":"694","publication_status":"published","has_accepted_license":"1","file":[{"relation":"main_file","success":1,"creator":"aehrmann","date_created":"2021-01-03T18:13:31Z","file_name":"_2021_Sölmann_AIMSBioneng8_25-35.pdf","access_level":"open_access","file_id":"695","content_type":"application/pdf","file_size":1154128,"date_updated":"2021-01-03T18:13:31Z"}],"department":[{"_id":"103"}],"page":"25-35","author":[{"full_name":"Sölmann, Sven","first_name":"Sven","last_name":"Sölmann"},{"first_name":"Anke","full_name":"Rattenholl, Anke","last_name":"Rattenholl"},{"first_name":"Hannah","full_name":"Blattner, Hannah","last_name":"Blattner"},{"full_name":"Ehrmann, Guido","first_name":"Guido","last_name":"Ehrmann"},{"last_name":"Gudermann","first_name":"Frank","full_name":"Gudermann, Frank"},{"full_name":"Lütkemeyer, Dirk","first_name":"Dirk","last_name":"Lütkemeyer"},{"orcid":"0000-0003-0695-3905","id":"223776","last_name":"Ehrmann","full_name":"Ehrmann, Andrea","orcid_put_code_url":"https://api.orcid.org/v2.0/0000-0003-0695-3905/work/105572547","first_name":"Andrea"}],"citation":{"ama":"Sölmann S, Rattenholl A, Blattner H, et al. Mammalian cell adhesion on different 3D printed polymers with varying sterilization methods and acidic treatment. AIMS Bioengineering. 2021;8(1):25-35.","mla":"Sölmann, Sven, et al. “ Mammalian Cell Adhesion on Different 3D Printed Polymers with Varying Sterilization Methods and Acidic Treatment.” AIMS Bioengineering, vol. 8, no. 1, 2021, pp. 25–35.","apa":"Sölmann, S., Rattenholl, A., Blattner, H., Ehrmann, G., Gudermann, F., Lütkemeyer, D., & Ehrmann, A. (2021). Mammalian cell adhesion on different 3D printed polymers with varying sterilization methods and acidic treatment. AIMS Bioengineering, 8(1), 25–35.","short":"S. Sölmann, A. Rattenholl, H. Blattner, G. Ehrmann, F. Gudermann, D. Lütkemeyer, A. Ehrmann, AIMS Bioengineering 8 (2021) 25–35.","ieee":"S. Sölmann et al., “ Mammalian cell adhesion on different 3D printed polymers with varying sterilization methods and acidic treatment,” AIMS Bioengineering, vol. 8, no. 1, pp. 25–35, 2021.","bibtex":"@article{Sölmann_Rattenholl_Blattner_Ehrmann_Gudermann_Lütkemeyer_Ehrmann_2021, title={ Mammalian cell adhesion on different 3D printed polymers with varying sterilization methods and acidic treatment}, volume={8}, number={1}, journal={AIMS Bioengineering}, author={Sölmann, Sven and Rattenholl, Anke and Blattner, Hannah and Ehrmann, Guido and Gudermann, Frank and Lütkemeyer, Dirk and Ehrmann, Andrea}, year={2021}, pages={25–35} }","chicago":"Sölmann, Sven, Anke Rattenholl, Hannah Blattner, Guido Ehrmann, Frank Gudermann, Dirk Lütkemeyer, and Andrea Ehrmann. “ Mammalian Cell Adhesion on Different 3D Printed Polymers with Varying Sterilization Methods and Acidic Treatment.” AIMS Bioengineering 8, no. 1 (2021): 25–35.","alphadin":"Sölmann, Sven ; Rattenholl, Anke ; Blattner, Hannah ; Ehrmann, Guido ; Gudermann, Frank ; Lütkemeyer, Dirk ; Ehrmann, Andrea: Mammalian cell adhesion on different 3D printed polymers with varying sterilization methods and acidic treatment. In: AIMS Bioengineering Bd. 8 (2021), Nr. 1, S. 25–35"},"language":[{"iso":"eng"}]},{"language":[{"iso":"eng"}],"doi":"10.3934/bioeng.2020004","ddc":["620"],"page":"43-54","author":[{"last_name":"Wehlage","first_name":"Daria","full_name":"Wehlage, Daria"},{"full_name":"Blattner, Hannah","first_name":"Hannah","last_name":"Blattner"},{"last_name":"Mamun","full_name":"Mamun, Al","first_name":"Al"},{"last_name":"Kutzli","full_name":"Kutzli, Ines","first_name":"Ines"},{"last_name":"Diestelhorst","first_name":"Elise","full_name":"Diestelhorst, Elise"},{"last_name":"Rattenholl","full_name":"Rattenholl, Anke","first_name":"Anke"},{"first_name":"Frank","full_name":"Gudermann, Frank","last_name":"Gudermann"},{"first_name":"Dirk","full_name":"Lütkemeyer, Dirk","last_name":"Lütkemeyer"},{"first_name":"Andrea","full_name":"Ehrmann, Andrea","last_name":"Ehrmann"}],"publication_identifier":{"issn":["2375-1495"]},"file":[{"date_created":"2021-01-03T15:00:59Z","relation":"main_file","creator":"aehrmann","success":1,"date_updated":"2021-01-03T15:00:59Z","file_size":1118818,"content_type":"application/pdf","file_id":"652","access_level":"open_access","file_name":"_2020_Wehlage_AIMSbioeng7_43-54.pdf"}],"department":[{"_id":"103"}],"_id":"651","publication_status":"published","has_accepted_license":"1","title":"Cell growth on electrospun nanofiber mats from polyacrylonitrile (PAN) blends","date_created":"2021-01-03T15:01:56Z","file_date_updated":"2021-01-03T15:00:59Z","status":"public","abstract":[{"lang":"eng","text":" Nanofiber mats can be produced by electrospinning from diverse polymers and polymer blends as well as with embedded ceramics, metals, etc. The large surface-to-volume ratio makes such nanofiber mats a well-suited substrate for tissue engineering and other cell growth experiments. Cell growth, however, is not only influenced by the substrate morphology, but also by the sterilization process applied before the experiment as well as by the chemical composition of the fibers. A former study showed that cell growth and adhesion are supported by polyacrylonitrile/gelatin nanofiber mats, while both factors are strongly reduced on pure polyacrylonitrile (PAN) nanofibers. Here we report on the influence of different PAN blends on cell growth and adhesion. Our study shows that adding ZnO to the PAN spinning solution impedes cell growth, while addition of maltodextrin/pea protein or casein/gelatin supports cell growth and adhesion."}],"date_updated":"2021-01-18T15:32:28Z","article_type":"original","issue":"1","volume":7,"user_id":"223776","publication":"AIMS Bioengineering","quality_controlled":"1","funded_apc":"1","type":"journal_article","oa":"1","intvolume":" 7","year":"2020","keyword":["electrospinning","nanofiber mat","autoclaving","cell growth","adherent cells","CHO cells","DMSO"]},{"quality_controlled":"1","type":"journal_article","oa":"1","intvolume":" 62","keyword":["polyacrylonitrile/gelatine nanofibrous mats","sterilization","autoclaving","ozone","UV sterilization","heat sterilization","cell growth","adherent cells","CHO cells","tissue engineering"],"year":"2019","date_updated":"2024-03-27T14:01:13Z","article_type":"original","issue":"2","volume":62,"user_id":"237837","publication":"Tekstilec ","urn":"urn:nbn:de:hbz:bi10-1661","_id":"166","has_accepted_license":"1","date_created":"2019-04-17T11:51:01Z","title":"Sterilization of PAN/Gelatin Nanofibrous Mats for Cell Growth","file_date_updated":"2019-05-28T06:52:24Z","status":"public","abstract":[{"lang":"eng","text":"Nanofi brous mats can be used as a substrate for eukaryotic cell growth in biotechnology, tissue engineering, etc. Several adherent cells (e.g. human fibroblasts) have been shown to grow well on fine fibres. For most applications, it is necessary to sterilize nanofibrous mats before adding the cells. Another possibility would be the addition of antibiotics and antimycotics to the cell culture medium to prevent microbial infection. However, antibiotics are disadvantageous since they might promote the growth of resistant bacteria in possible future medical applications of nanofibrous mats. Possible sterilization techniques include autoclaving, UV-sterilization, ozone treatment, heat sterilization and other techniques which usually necessitate more expensive equipment, such as gamma irradiation. Systematic examinations of the infl uence of different sterilization techniques on the cell growth on nanofibrous mats have not yet been reported in the literature. Here, we report on the first experimental investigations of the effect of sterilization with different methods on the properties of polyacrylonitrile (PAN)/gelatine nanofibrous mats, and the resulting growth\r\nand adhesion of Chinese hamster ovary cells. While all techniques under investigation yielded sterile nanofibrous mats, autoclaving and heat sterilization change the PAN/gelatine fibre morphology. Ozone, on the other hand, modifies the pH value of the culture medium and partly impedes cell adhesion. UV sterilization also suggests a chemical modification of the nanofibrous mat. Unexpectedly, heat sterilization resulted in the highest amount of adherent Chinese hamster ovary cells grown on PAN/gelatine nanofibrous mats in spite of gelatine melting."}],"language":[{"iso":"eng"}],"citation":{"chicago":"Wehlage, Daria, Hannah Blattner, Lilia Sabantina, Robin Böttjer, Timo Grothe, Anke Rattenholl, Frank Gudermann, Dirk Lütkemeyer, and Andrea Ehrmann. “Sterilization of PAN/Gelatin Nanofibrous Mats for Cell Growth.” Tekstilec 62, no. 2 (2019): 78–88. https://doi.org/10.14502/Tekstilec2019.62.78-88.","alphadin":"Wehlage, Daria ; Blattner, Hannah ; Sabantina, Lilia ; Böttjer, Robin ; Grothe, Timo ; Rattenholl, Anke ; Gudermann, Frank ; Lütkemeyer, Dirk ; u. a.: Sterilization of PAN/Gelatin Nanofibrous Mats for Cell Growth. In: Tekstilec Bd. 62 (2019), Nr. 2, S. 78–88","ieee":"D. Wehlage et al., “Sterilization of PAN/Gelatin Nanofibrous Mats for Cell Growth,” Tekstilec , vol. 62, no. 2, pp. 78–88, 2019.","bibtex":"@article{Wehlage_Blattner_Sabantina_Böttjer_Grothe_Rattenholl_Gudermann_Lütkemeyer_Ehrmann_2019, title={Sterilization of PAN/Gelatin Nanofibrous Mats for Cell Growth}, volume={62}, DOI={10.14502/Tekstilec2019.62.78-88}, number={2}, journal={Tekstilec }, author={Wehlage, Daria and Blattner, Hannah and Sabantina, Lilia and Böttjer, Robin and Grothe, Timo and Rattenholl, Anke and Gudermann, Frank and Lütkemeyer, Dirk and Ehrmann, Andrea}, year={2019}, pages={78–88} }","apa":"Wehlage, D., Blattner, H., Sabantina, L., Böttjer, R., Grothe, T., Rattenholl, A., … Ehrmann, A. (2019). Sterilization of PAN/Gelatin Nanofibrous Mats for Cell Growth. Tekstilec , 62(2), 78–88. https://doi.org/10.14502/Tekstilec2019.62.78-88","short":"D. Wehlage, H. Blattner, L. Sabantina, R. Böttjer, T. Grothe, A. Rattenholl, F. Gudermann, D. Lütkemeyer, A. Ehrmann, Tekstilec 62 (2019) 78–88.","ama":"Wehlage D, Blattner H, Sabantina L, et al. Sterilization of PAN/Gelatin Nanofibrous Mats for Cell Growth. Tekstilec . 2019;62(2):78-88. doi:10.14502/Tekstilec2019.62.78-88","mla":"Wehlage, Daria, et al. “Sterilization of PAN/Gelatin Nanofibrous Mats for Cell Growth.” Tekstilec , vol. 62, no. 2, 2019, pp. 78–88, doi:10.14502/Tekstilec2019.62.78-88."},"doi":"10.14502/Tekstilec2019.62.78-88","page":"78-88","author":[{"full_name":"Wehlage, Daria","first_name":"Daria","last_name":"Wehlage"},{"last_name":"Blattner","first_name":"Hannah","full_name":"Blattner, Hannah"},{"first_name":"Lilia","full_name":"Sabantina, Lilia","last_name":"Sabantina"},{"last_name":"Böttjer","full_name":"Böttjer, Robin","first_name":"Robin"},{"id":"221330","orcid":"0000-0002-9099-4277","last_name":"Grothe","first_name":"Timo","full_name":"Grothe, Timo","orcid_put_code_url":"https://api.orcid.org/v2.0/0000-0002-9099-4277/work/94763802"},{"last_name":"Rattenholl","first_name":"Anke","full_name":"Rattenholl, Anke"},{"first_name":"Frank","full_name":"Gudermann, Frank","last_name":"Gudermann"},{"last_name":"Lütkemeyer","first_name":"Dirk","full_name":"Lütkemeyer, Dirk"},{"full_name":"Ehrmann, Andrea","orcid_put_code_url":"https://api.orcid.org/v2.0/0000-0003-0695-3905/work/94763803","first_name":"Andrea","last_name":"Ehrmann","orcid":"0000-0003-0695-3905","id":"223776"}],"file":[{"access_level":"open_access","file_id":"274","file_name":"_2019_Wehlage_Tekstilec62_78-88.pdf","date_updated":"2019-05-28T06:52:24Z","file_size":959760,"content_type":"application/pdf","relation":"main_file","creator":"mstock1","success":1,"date_created":"2019-05-28T06:52:24Z"}]}]