--- _id: '694' abstract: - lang: eng text: ' Cell growth on three-dimensional objects is of high interest for bioprocess engineering of adherent cells, tissue engineering and other biomedical applications. 3D printing by fusion deposition modeling (FDM) is a cheap possibility to generate tailor-made substrates for cell growth. Nevertheless, not all materials are chemically attractive for cells. Polylactic acid (PLA) and polyethylene terephthalate glycol (PETG) have been reported to be suitable polymers for tissue engineering. Thus, they might also be applicable for cost-effective bioprocessing of adherent cell lines. Here we report on the influence of printing material, surface structure, and sterilization method on Chinese hamster ovary (CHO) cell adhesion on a modified, high temperature resistant PLA, a PETG blend, and unmodified PETG, respectively. Our study revealed that CHO cells grew on all polymers tested without further surface modification. Samples could be efficiently chemically sterilized. Additional acid treatment had no significant effect on cell adhesion. ' article_type: original author: - first_name: Sven full_name: Sölmann, Sven last_name: Sölmann - first_name: Anke full_name: Rattenholl, Anke last_name: Rattenholl - first_name: Hannah full_name: Blattner, Hannah last_name: Blattner - first_name: Guido full_name: Ehrmann, Guido last_name: Ehrmann - first_name: Frank full_name: Gudermann, Frank last_name: Gudermann - first_name: Dirk full_name: Lütkemeyer, Dirk last_name: Lütkemeyer - first_name: Andrea full_name: Ehrmann, Andrea id: '223776' last_name: Ehrmann orcid: 0000-0003-0695-3905 orcid_put_code_url: https://api.orcid.org/v2.0/0000-0003-0695-3905/work/105572547 citation: alphadin: 'Sölmann, Sven ; Rattenholl, Anke ; Blattner, Hannah ; Ehrmann, Guido ; Gudermann, Frank ; Lütkemeyer, Dirk ; Ehrmann, Andrea: Mammalian cell adhesion on different 3D printed polymers with varying sterilization methods and acidic treatment. In: AIMS Bioengineering Bd. 8 (2021), Nr. 1, S. 25–35' ama: Sölmann S, Rattenholl A, Blattner H, et al. Mammalian cell adhesion on different 3D printed polymers with varying sterilization methods and acidic treatment. AIMS Bioengineering. 2021;8(1):25-35. apa: Sölmann, S., Rattenholl, A., Blattner, H., Ehrmann, G., Gudermann, F., Lütkemeyer, D., & Ehrmann, A. (2021). Mammalian cell adhesion on different 3D printed polymers with varying sterilization methods and acidic treatment. AIMS Bioengineering, 8(1), 25–35. bibtex: '@article{Sölmann_Rattenholl_Blattner_Ehrmann_Gudermann_Lütkemeyer_Ehrmann_2021, title={ Mammalian cell adhesion on different 3D printed polymers with varying sterilization methods and acidic treatment}, volume={8}, number={1}, journal={AIMS Bioengineering}, author={Sölmann, Sven and Rattenholl, Anke and Blattner, Hannah and Ehrmann, Guido and Gudermann, Frank and Lütkemeyer, Dirk and Ehrmann, Andrea}, year={2021}, pages={25–35} }' chicago: 'Sölmann, Sven, Anke Rattenholl, Hannah Blattner, Guido Ehrmann, Frank Gudermann, Dirk Lütkemeyer, and Andrea Ehrmann. “ Mammalian Cell Adhesion on Different 3D Printed Polymers with Varying Sterilization Methods and Acidic Treatment.” AIMS Bioengineering 8, no. 1 (2021): 25–35.' ieee: S. Sölmann et al., “ Mammalian cell adhesion on different 3D printed polymers with varying sterilization methods and acidic treatment,” AIMS Bioengineering, vol. 8, no. 1, pp. 25–35, 2021. mla: Sölmann, Sven, et al. “ Mammalian Cell Adhesion on Different 3D Printed Polymers with Varying Sterilization Methods and Acidic Treatment.” AIMS Bioengineering, vol. 8, no. 1, 2021, pp. 25–35. short: S. Sölmann, A. Rattenholl, H. Blattner, G. Ehrmann, F. Gudermann, D. Lütkemeyer, A. Ehrmann, AIMS Bioengineering 8 (2021) 25–35. date_created: 2021-01-03T18:14:05Z date_updated: 2022-01-01T15:23:09Z department: - _id: '103' file: - access_level: open_access content_type: application/pdf creator: aehrmann date_created: 2021-01-03T18:13:31Z date_updated: 2021-01-03T18:13:31Z file_id: '695' file_name: _2021_Sölmann_AIMSBioneng8_25-35.pdf file_size: 1154128 relation: main_file success: 1 file_date_updated: 2021-01-03T18:13:31Z funded_apc: '1' has_accepted_license: '1' intvolume: ' 8' issue: '1' keyword: - 3D printing - FDM printing - sterilization - adherent cells - CHO cells language: - iso: eng main_file_link: - open_access: '1' oa: '1' page: 25-35 publication: AIMS Bioengineering publication_status: published quality_controlled: '1' status: public title: ' Mammalian cell adhesion on different 3D printed polymers with varying sterilization methods and acidic treatment' type: journal_article user_id: '223776' volume: 8 year: '2021' ... --- _id: '1598' abstract: - lang: eng text: " In biotechnology, the field of cell cultivation is highly relevant. Cultivated cells can be used, for example, for the development of biopharmaceuticals and in tissue engineering. Commonly, mammalian cells are grown in bioreactors, T-flasks, well plates, etc., without a specific substrate. Nanofibrous mats, however, have been reported to promote cell growth, adhesion, and proliferation. Here, we give an overview of the different attempts at cultivating mammalian cells on electrospun nanofiber mats for biotechnological and biomedical purposes. Starting with a brief overview of the different electrospinning methods, resulting in random or defined fiber orientations in the nanofiber mats, we describe the typical materials used in cell growth applications in biotechnology and tissue engineering. The influence of using different surface morphologies and polymers or polymer blends on the possible application of such nanofiber mats for tissue engineering and other biotechnological applications is discussed. Polymer blends, in particular, can often be used to reach the required combination of mechanical and biological properties, making such nanofiber mats highly suitable for tissue engineering and other biotechnological or biomedical cell growth applications.\r\n " article_number: '6929' article_type: review author: - first_name: Ewin full_name: Tanzli, Ewin last_name: Tanzli - first_name: Andrea full_name: Ehrmann, Andrea id: '223776' last_name: Ehrmann orcid: 0000-0003-0695-3905 orcid_put_code_url: https://api.orcid.org/v2.0/0000-0003-0695-3905/work/105572124 citation: alphadin: 'Tanzli, Ewin ; Ehrmann, Andrea: Electrospun Nanofibrous Membranes for Tissue Engineering and Cell Growth. In: Applied Sciences Bd. 11, MDPI AG (2021), Nr. 15' ama: Tanzli E, Ehrmann A. Electrospun Nanofibrous Membranes for Tissue Engineering and Cell Growth. Applied Sciences. 2021;11(15). doi:10.3390/app11156929 apa: Tanzli, E., & Ehrmann, A. (2021). Electrospun Nanofibrous Membranes for Tissue Engineering and Cell Growth. Applied Sciences, 11(15). https://doi.org/10.3390/app11156929 bibtex: '@article{Tanzli_Ehrmann_2021, title={Electrospun Nanofibrous Membranes for Tissue Engineering and Cell Growth}, volume={11}, DOI={10.3390/app11156929}, number={156929}, journal={Applied Sciences}, publisher={MDPI AG}, author={Tanzli, Ewin and Ehrmann, Andrea}, year={2021} }' chicago: Tanzli, Ewin, and Andrea Ehrmann. “Electrospun Nanofibrous Membranes for Tissue Engineering and Cell Growth.” Applied Sciences 11, no. 15 (2021). https://doi.org/10.3390/app11156929. ieee: E. Tanzli and A. Ehrmann, “Electrospun Nanofibrous Membranes for Tissue Engineering and Cell Growth,” Applied Sciences, vol. 11, no. 15, 2021. mla: Tanzli, Ewin, and Andrea Ehrmann. “Electrospun Nanofibrous Membranes for Tissue Engineering and Cell Growth.” Applied Sciences, vol. 11, no. 15, 6929, MDPI AG, 2021, doi:10.3390/app11156929. short: E. Tanzli, A. Ehrmann, Applied Sciences 11 (2021). date_created: 2022-01-01T13:57:36Z date_updated: 2022-01-01T15:11:15Z department: - _id: '103' doi: 10.3390/app11156929 intvolume: ' 11' issue: '15' keyword: - cell growth - electrospinning - nanofibrous membrane - adherent cells - biomedicine language: - iso: eng main_file_link: - open_access: '1' url: https://doi.org/10.3390/app11156929 oa: '1' publication: Applied Sciences publication_identifier: eissn: - 2076-3417 publication_status: published publisher: MDPI AG quality_controlled: '1' status: public title: Electrospun Nanofibrous Membranes for Tissue Engineering and Cell Growth tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: '223776' volume: 11 year: '2021' ... --- _id: '651' abstract: - lang: eng text: ' Nanofiber mats can be produced by electrospinning from diverse polymers and polymer blends as well as with embedded ceramics, metals, etc. The large surface-to-volume ratio makes such nanofiber mats a well-suited substrate for tissue engineering and other cell growth experiments. Cell growth, however, is not only influenced by the substrate morphology, but also by the sterilization process applied before the experiment as well as by the chemical composition of the fibers. A former study showed that cell growth and adhesion are supported by polyacrylonitrile/gelatin nanofiber mats, while both factors are strongly reduced on pure polyacrylonitrile (PAN) nanofibers. Here we report on the influence of different PAN blends on cell growth and adhesion. Our study shows that adding ZnO to the PAN spinning solution impedes cell growth, while addition of maltodextrin/pea protein or casein/gelatin supports cell growth and adhesion.' article_type: original author: - first_name: Daria full_name: Wehlage, Daria last_name: Wehlage - first_name: Hannah full_name: Blattner, Hannah last_name: Blattner - first_name: Al full_name: Mamun, Al last_name: Mamun - first_name: Ines full_name: Kutzli, Ines last_name: Kutzli - first_name: Elise full_name: Diestelhorst, Elise last_name: Diestelhorst - first_name: Anke full_name: Rattenholl, Anke last_name: Rattenholl - first_name: Frank full_name: Gudermann, Frank last_name: Gudermann - first_name: Dirk full_name: Lütkemeyer, Dirk last_name: Lütkemeyer - first_name: Andrea full_name: Ehrmann, Andrea last_name: Ehrmann date_created: 2021-01-03T15:01:56Z date_updated: 2021-01-18T15:32:28Z ddc: - '620' department: - _id: '103' doi: 10.3934/bioeng.2020004 file: - access_level: open_access content_type: application/pdf creator: aehrmann date_created: 2021-01-03T15:00:59Z date_updated: 2021-01-03T15:00:59Z file_id: '652' file_name: _2020_Wehlage_AIMSbioeng7_43-54.pdf file_size: 1118818 relation: main_file success: 1 file_date_updated: 2021-01-03T15:00:59Z funded_apc: '1' has_accepted_license: '1' intvolume: ' 7' issue: '1' keyword: - electrospinning - nanofiber mat - autoclaving - cell growth - adherent cells - CHO cells - DMSO language: - iso: eng oa: '1' page: 43-54 publication: AIMS Bioengineering publication_identifier: issn: - 2375-1495 publication_status: published quality_controlled: '1' status: public title: Cell growth on electrospun nanofiber mats from polyacrylonitrile (PAN) blends type: journal_article user_id: '223776' volume: 7 year: '2020' ... --- _id: '166' abstract: - lang: eng text: "Nanofi brous mats can be used as a substrate for eukaryotic cell growth in biotechnology, tissue engineering, etc. Several adherent cells (e.g. human fibroblasts) have been shown to grow well on fine fibres. For most applications, it is necessary to sterilize nanofibrous mats before adding the cells. Another possibility would be the addition of antibiotics and antimycotics to the cell culture medium to prevent microbial infection. However, antibiotics are disadvantageous since they might promote the growth of resistant bacteria in possible future medical applications of nanofibrous mats. Possible sterilization techniques include autoclaving, UV-sterilization, ozone treatment, heat sterilization and other techniques which usually necessitate more expensive equipment, such as gamma irradiation. Systematic examinations of the infl uence of different sterilization techniques on the cell growth on nanofibrous mats have not yet been reported in the literature. Here, we report on the first experimental investigations of the effect of sterilization with different methods on the properties of polyacrylonitrile (PAN)/gelatine nanofibrous mats, and the resulting growth\r\nand adhesion of Chinese hamster ovary cells. While all techniques under investigation yielded sterile nanofibrous mats, autoclaving and heat sterilization change the PAN/gelatine fibre morphology. Ozone, on the other hand, modifies the pH value of the culture medium and partly impedes cell adhesion. UV sterilization also suggests a chemical modification of the nanofibrous mat. Unexpectedly, heat sterilization resulted in the highest amount of adherent Chinese hamster ovary cells grown on PAN/gelatine nanofibrous mats in spite of gelatine melting." article_type: original author: - first_name: Daria full_name: Wehlage, Daria last_name: Wehlage - first_name: Hannah full_name: Blattner, Hannah last_name: Blattner - first_name: Lilia full_name: Sabantina, Lilia last_name: Sabantina - first_name: Robin full_name: Böttjer, Robin last_name: Böttjer - first_name: Timo full_name: Grothe, Timo id: '221330' last_name: Grothe orcid: 0000-0002-9099-4277 orcid_put_code_url: https://api.orcid.org/v2.0/0000-0002-9099-4277/work/94763802 - first_name: Anke full_name: Rattenholl, Anke last_name: Rattenholl - first_name: Frank full_name: Gudermann, Frank last_name: Gudermann - first_name: Dirk full_name: Lütkemeyer, Dirk last_name: Lütkemeyer - first_name: Andrea full_name: Ehrmann, Andrea id: '223776' last_name: Ehrmann orcid: 0000-0003-0695-3905 orcid_put_code_url: https://api.orcid.org/v2.0/0000-0003-0695-3905/work/94763803 citation: alphadin: 'Wehlage, Daria ; Blattner, Hannah ; Sabantina, Lilia ; Böttjer, Robin ; Grothe, Timo ; Rattenholl, Anke ; Gudermann, Frank ; Lütkemeyer, Dirk ; u. a.: Sterilization of PAN/Gelatin Nanofibrous Mats for Cell Growth. In: Tekstilec Bd. 62 (2019), Nr. 2, S. 78–88' ama: Wehlage D, Blattner H, Sabantina L, et al. Sterilization of PAN/Gelatin Nanofibrous Mats for Cell Growth. Tekstilec . 2019;62(2):78-88. doi:10.14502/Tekstilec2019.62.78-88 apa: Wehlage, D., Blattner, H., Sabantina, L., Böttjer, R., Grothe, T., Rattenholl, A., … Ehrmann, A. (2019). Sterilization of PAN/Gelatin Nanofibrous Mats for Cell Growth. Tekstilec , 62(2), 78–88. https://doi.org/10.14502/Tekstilec2019.62.78-88 bibtex: '@article{Wehlage_Blattner_Sabantina_Böttjer_Grothe_Rattenholl_Gudermann_Lütkemeyer_Ehrmann_2019, title={Sterilization of PAN/Gelatin Nanofibrous Mats for Cell Growth}, volume={62}, DOI={10.14502/Tekstilec2019.62.78-88}, number={2}, journal={Tekstilec }, author={Wehlage, Daria and Blattner, Hannah and Sabantina, Lilia and Böttjer, Robin and Grothe, Timo and Rattenholl, Anke and Gudermann, Frank and Lütkemeyer, Dirk and Ehrmann, Andrea}, year={2019}, pages={78–88} }' chicago: 'Wehlage, Daria, Hannah Blattner, Lilia Sabantina, Robin Böttjer, Timo Grothe, Anke Rattenholl, Frank Gudermann, Dirk Lütkemeyer, and Andrea Ehrmann. “Sterilization of PAN/Gelatin Nanofibrous Mats for Cell Growth.” Tekstilec 62, no. 2 (2019): 78–88. https://doi.org/10.14502/Tekstilec2019.62.78-88.' ieee: D. Wehlage et al., “Sterilization of PAN/Gelatin Nanofibrous Mats for Cell Growth,” Tekstilec , vol. 62, no. 2, pp. 78–88, 2019. mla: Wehlage, Daria, et al. “Sterilization of PAN/Gelatin Nanofibrous Mats for Cell Growth.” Tekstilec , vol. 62, no. 2, 2019, pp. 78–88, doi:10.14502/Tekstilec2019.62.78-88. short: D. Wehlage, H. Blattner, L. Sabantina, R. Böttjer, T. Grothe, A. Rattenholl, F. Gudermann, D. Lütkemeyer, A. Ehrmann, Tekstilec 62 (2019) 78–88. date_created: 2019-04-17T11:51:01Z date_updated: 2024-03-27T14:01:13Z doi: 10.14502/Tekstilec2019.62.78-88 file: - access_level: open_access content_type: application/pdf creator: mstock1 date_created: 2019-05-28T06:52:24Z date_updated: 2019-05-28T06:52:24Z file_id: '274' file_name: _2019_Wehlage_Tekstilec62_78-88.pdf file_size: 959760 relation: main_file success: 1 file_date_updated: 2019-05-28T06:52:24Z has_accepted_license: '1' intvolume: ' 62' issue: '2' keyword: - polyacrylonitrile/gelatine nanofibrous mats - sterilization - autoclaving - ozone - UV sterilization - heat sterilization - cell growth - adherent cells - CHO cells - tissue engineering language: - iso: eng oa: '1' page: 78-88 publication: 'Tekstilec ' quality_controlled: '1' status: public title: Sterilization of PAN/Gelatin Nanofibrous Mats for Cell Growth type: journal_article urn: urn:nbn:de:hbz:bi10-1661 user_id: '237837' volume: 62 year: '2019' ...