TY - JOUR AB - Nanofiber mats can be produced by electrospinning from diverse polymers and polymer blends as well as with embedded ceramics, metals, etc. The large surface-to-volume ratio makes such nanofiber mats a well-suited substrate for tissue engineering and other cell growth experiments. Cell growth, however, is not only influenced by the substrate morphology, but also by the sterilization process applied before the experiment as well as by the chemical composition of the fibers. A former study showed that cell growth and adhesion are supported by polyacrylonitrile/gelatin nanofiber mats, while both factors are strongly reduced on pure polyacrylonitrile (PAN) nanofibers. Here we report on the influence of different PAN blends on cell growth and adhesion. Our study shows that adding ZnO to the PAN spinning solution impedes cell growth, while addition of maltodextrin/pea protein or casein/gelatin supports cell growth and adhesion. AU - Wehlage, Daria AU - Blattner, Hannah AU - Mamun, Al AU - Kutzli, Ines AU - Diestelhorst, Elise AU - Rattenholl, Anke AU - Gudermann, Frank AU - Lütkemeyer, Dirk AU - Ehrmann, Andrea ID - 651 IS - 1 JF - AIMS Bioengineering KW - electrospinning KW - nanofiber mat KW - autoclaving KW - cell growth KW - adherent cells KW - CHO cells KW - DMSO SN - 2375-1495 TI - Cell growth on electrospun nanofiber mats from polyacrylonitrile (PAN) blends VL - 7 ER - TY - JOUR AB - Nanofi brous mats can be used as a substrate for eukaryotic cell growth in biotechnology, tissue engineering, etc. Several adherent cells (e.g. human fibroblasts) have been shown to grow well on fine fibres. For most applications, it is necessary to sterilize nanofibrous mats before adding the cells. Another possibility would be the addition of antibiotics and antimycotics to the cell culture medium to prevent microbial infection. However, antibiotics are disadvantageous since they might promote the growth of resistant bacteria in possible future medical applications of nanofibrous mats. Possible sterilization techniques include autoclaving, UV-sterilization, ozone treatment, heat sterilization and other techniques which usually necessitate more expensive equipment, such as gamma irradiation. Systematic examinations of the infl uence of different sterilization techniques on the cell growth on nanofibrous mats have not yet been reported in the literature. Here, we report on the first experimental investigations of the effect of sterilization with different methods on the properties of polyacrylonitrile (PAN)/gelatine nanofibrous mats, and the resulting growth and adhesion of Chinese hamster ovary cells. While all techniques under investigation yielded sterile nanofibrous mats, autoclaving and heat sterilization change the PAN/gelatine fibre morphology. Ozone, on the other hand, modifies the pH value of the culture medium and partly impedes cell adhesion. UV sterilization also suggests a chemical modification of the nanofibrous mat. Unexpectedly, heat sterilization resulted in the highest amount of adherent Chinese hamster ovary cells grown on PAN/gelatine nanofibrous mats in spite of gelatine melting. AU - Wehlage, Daria AU - Blattner, Hannah AU - Sabantina, Lilia AU - Böttjer, Robin AU - Grothe, Timo AU - Rattenholl, Anke AU - Gudermann, Frank AU - Lütkemeyer, Dirk AU - Ehrmann, Andrea ID - 166 IS - 2 JF - Tekstilec KW - polyacrylonitrile/gelatine nanofibrous mats KW - sterilization KW - autoclaving KW - ozone KW - UV sterilization KW - heat sterilization KW - cell growth KW - adherent cells KW - CHO cells KW - tissue engineering TI - Sterilization of PAN/Gelatin Nanofibrous Mats for Cell Growth VL - 62 ER -