@article{1598, abstract = { In biotechnology, the field of cell cultivation is highly relevant. Cultivated cells can be used, for example, for the development of biopharmaceuticals and in tissue engineering. Commonly, mammalian cells are grown in bioreactors, T-flasks, well plates, etc., without a specific substrate. Nanofibrous mats, however, have been reported to promote cell growth, adhesion, and proliferation. Here, we give an overview of the different attempts at cultivating mammalian cells on electrospun nanofiber mats for biotechnological and biomedical purposes. Starting with a brief overview of the different electrospinning methods, resulting in random or defined fiber orientations in the nanofiber mats, we describe the typical materials used in cell growth applications in biotechnology and tissue engineering. The influence of using different surface morphologies and polymers or polymer blends on the possible application of such nanofiber mats for tissue engineering and other biotechnological applications is discussed. Polymer blends, in particular, can often be used to reach the required combination of mechanical and biological properties, making such nanofiber mats highly suitable for tissue engineering and other biotechnological or biomedical cell growth applications. }, author = {Tanzli, Ewin and Ehrmann, Andrea}, issn = {2076-3417}, journal = {Applied Sciences}, keywords = {cell growth, electrospinning, nanofibrous membrane, adherent cells, biomedicine}, number = {15}, publisher = {MDPI AG}, title = {{Electrospun Nanofibrous Membranes for Tissue Engineering and Cell Growth}}, doi = {10.3390/app11156929}, volume = {11}, year = {2021}, } @article{651, abstract = { Nanofiber mats can be produced by electrospinning from diverse polymers and polymer blends as well as with embedded ceramics, metals, etc. The large surface-to-volume ratio makes such nanofiber mats a well-suited substrate for tissue engineering and other cell growth experiments. Cell growth, however, is not only influenced by the substrate morphology, but also by the sterilization process applied before the experiment as well as by the chemical composition of the fibers. A former study showed that cell growth and adhesion are supported by polyacrylonitrile/gelatin nanofiber mats, while both factors are strongly reduced on pure polyacrylonitrile (PAN) nanofibers. Here we report on the influence of different PAN blends on cell growth and adhesion. Our study shows that adding ZnO to the PAN spinning solution impedes cell growth, while addition of maltodextrin/pea protein or casein/gelatin supports cell growth and adhesion.}, author = {Wehlage, Daria and Blattner, Hannah and Mamun, Al and Kutzli, Ines and Diestelhorst, Elise and Rattenholl, Anke and Gudermann, Frank and Lütkemeyer, Dirk and Ehrmann, Andrea}, issn = {2375-1495}, journal = {AIMS Bioengineering}, keywords = {electrospinning, nanofiber mat, autoclaving, cell growth, adherent cells, CHO cells, DMSO}, number = {1}, pages = {43--54}, title = {{Cell growth on electrospun nanofiber mats from polyacrylonitrile (PAN) blends}}, doi = {10.3934/bioeng.2020004}, volume = {7}, year = {2020}, } @article{166, abstract = {Nanofi brous mats can be used as a substrate for eukaryotic cell growth in biotechnology, tissue engineering, etc. Several adherent cells (e.g. human fibroblasts) have been shown to grow well on fine fibres. For most applications, it is necessary to sterilize nanofibrous mats before adding the cells. Another possibility would be the addition of antibiotics and antimycotics to the cell culture medium to prevent microbial infection. However, antibiotics are disadvantageous since they might promote the growth of resistant bacteria in possible future medical applications of nanofibrous mats. Possible sterilization techniques include autoclaving, UV-sterilization, ozone treatment, heat sterilization and other techniques which usually necessitate more expensive equipment, such as gamma irradiation. Systematic examinations of the infl uence of different sterilization techniques on the cell growth on nanofibrous mats have not yet been reported in the literature. Here, we report on the first experimental investigations of the effect of sterilization with different methods on the properties of polyacrylonitrile (PAN)/gelatine nanofibrous mats, and the resulting growth and adhesion of Chinese hamster ovary cells. While all techniques under investigation yielded sterile nanofibrous mats, autoclaving and heat sterilization change the PAN/gelatine fibre morphology. Ozone, on the other hand, modifies the pH value of the culture medium and partly impedes cell adhesion. UV sterilization also suggests a chemical modification of the nanofibrous mat. Unexpectedly, heat sterilization resulted in the highest amount of adherent Chinese hamster ovary cells grown on PAN/gelatine nanofibrous mats in spite of gelatine melting.}, author = {Wehlage, Daria and Blattner, Hannah and Sabantina, Lilia and Böttjer, Robin and Grothe, Timo and Rattenholl, Anke and Gudermann, Frank and Lütkemeyer, Dirk and Ehrmann, Andrea}, journal = {Tekstilec }, keywords = {polyacrylonitrile/gelatine nanofibrous mats, sterilization, autoclaving, ozone, UV sterilization, heat sterilization, cell growth, adherent cells, CHO cells, tissue engineering}, number = {2}, pages = {78--88}, title = {{Sterilization of PAN/Gelatin Nanofibrous Mats for Cell Growth}}, doi = {10.14502/Tekstilec2019.62.78-88}, volume = {62}, year = {2019}, }