---
_id: '1598'
abstract:
- lang: eng
text: " In biotechnology, the field of cell cultivation is highly
relevant. Cultivated cells can be used, for example, for the development of biopharmaceuticals
and in tissue engineering. Commonly, mammalian cells are grown in bioreactors,
T-flasks, well plates, etc., without a specific substrate. Nanofibrous mats, however,
have been reported to promote cell growth, adhesion, and proliferation. Here,
we give an overview of the different attempts at cultivating mammalian cells on
electrospun nanofiber mats for biotechnological and biomedical purposes. Starting
with a brief overview of the different electrospinning methods, resulting in random
or defined fiber orientations in the nanofiber mats, we describe the typical materials
used in cell growth applications in biotechnology and tissue engineering. The
influence of using different surface morphologies and polymers or polymer blends
on the possible application of such nanofiber mats for tissue engineering and
other biotechnological applications is discussed. Polymer blends, in particular,
can often be used to reach the required combination of mechanical and biological
properties, making such nanofiber mats highly suitable for tissue engineering
and other biotechnological or biomedical cell growth applications.\r\n "
article_number: '6929'
article_type: review
author:
- first_name: Ewin
full_name: Tanzli, Ewin
last_name: Tanzli
- first_name: Andrea
full_name: Ehrmann, Andrea
id: '223776'
last_name: Ehrmann
orcid: 0000-0003-0695-3905
orcid_put_code_url: https://api.orcid.org/v2.0/0000-0003-0695-3905/work/105572124
citation:
alphadin: 'Tanzli, Ewin ; Ehrmann,
Andrea: Electrospun Nanofibrous Membranes for Tissue Engineering and Cell
Growth. In: Applied Sciences Bd. 11, MDPI AG (2021), Nr. 15'
ama: Tanzli E, Ehrmann A. Electrospun Nanofibrous Membranes for Tissue Engineering
and Cell Growth. Applied Sciences. 2021;11(15). doi:10.3390/app11156929
apa: Tanzli, E., & Ehrmann, A. (2021). Electrospun Nanofibrous Membranes for
Tissue Engineering and Cell Growth. Applied Sciences, 11(15). https://doi.org/10.3390/app11156929
bibtex: '@article{Tanzli_Ehrmann_2021, title={Electrospun Nanofibrous Membranes
for Tissue Engineering and Cell Growth}, volume={11}, DOI={10.3390/app11156929},
number={156929}, journal={Applied Sciences}, publisher={MDPI AG}, author={Tanzli,
Ewin and Ehrmann, Andrea}, year={2021} }'
chicago: Tanzli, Ewin, and Andrea Ehrmann. “Electrospun Nanofibrous Membranes for
Tissue Engineering and Cell Growth.” Applied Sciences 11, no. 15 (2021).
https://doi.org/10.3390/app11156929.
ieee: E. Tanzli and A. Ehrmann, “Electrospun Nanofibrous Membranes for Tissue Engineering
and Cell Growth,” Applied Sciences, vol. 11, no. 15, 2021.
mla: Tanzli, Ewin, and Andrea Ehrmann. “Electrospun Nanofibrous Membranes for Tissue
Engineering and Cell Growth.” Applied Sciences, vol. 11, no. 15, 6929,
MDPI AG, 2021, doi:10.3390/app11156929.
short: E. Tanzli, A. Ehrmann, Applied Sciences 11 (2021).
date_created: 2022-01-01T13:57:36Z
date_updated: 2022-01-01T15:11:15Z
department:
- _id: '103'
doi: 10.3390/app11156929
intvolume: ' 11'
issue: '15'
keyword:
- cell growth
- electrospinning
- nanofibrous membrane
- adherent cells
- biomedicine
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.3390/app11156929
oa: '1'
publication: Applied Sciences
publication_identifier:
eissn:
- 2076-3417
publication_status: published
publisher: MDPI AG
quality_controlled: '1'
status: public
title: Electrospun Nanofibrous Membranes for Tissue Engineering and Cell Growth
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: '223776'
volume: 11
year: '2021'
...
---
_id: '651'
abstract:
- lang: eng
text: ' Nanofiber mats can be produced by electrospinning from diverse polymers
and polymer blends as well as with embedded ceramics, metals, etc. The large surface-to-volume
ratio makes such nanofiber mats a well-suited substrate for tissue engineering
and other cell growth experiments. Cell growth, however, is not only influenced
by the substrate morphology, but also by the sterilization process applied before
the experiment as well as by the chemical composition of the fibers. A former
study showed that cell growth and adhesion are supported by polyacrylonitrile/gelatin
nanofiber mats, while both factors are strongly reduced on pure polyacrylonitrile
(PAN) nanofibers. Here we report on the influence of different PAN blends on cell
growth and adhesion. Our study shows that adding ZnO to the PAN spinning solution
impedes cell growth, while addition of maltodextrin/pea protein or casein/gelatin
supports cell growth and adhesion.'
article_type: original
author:
- first_name: Daria
full_name: Wehlage, Daria
last_name: Wehlage
- first_name: Hannah
full_name: Blattner, Hannah
last_name: Blattner
- first_name: Al
full_name: Mamun, Al
last_name: Mamun
- first_name: Ines
full_name: Kutzli, Ines
last_name: Kutzli
- first_name: Elise
full_name: Diestelhorst, Elise
last_name: Diestelhorst
- first_name: Anke
full_name: Rattenholl, Anke
last_name: Rattenholl
- first_name: Frank
full_name: Gudermann, Frank
last_name: Gudermann
- first_name: Dirk
full_name: Lütkemeyer, Dirk
last_name: Lütkemeyer
- first_name: Andrea
full_name: Ehrmann, Andrea
last_name: Ehrmann
date_created: 2021-01-03T15:01:56Z
date_updated: 2021-01-18T15:32:28Z
ddc:
- '620'
department:
- _id: '103'
doi: 10.3934/bioeng.2020004
file:
- access_level: open_access
content_type: application/pdf
creator: aehrmann
date_created: 2021-01-03T15:00:59Z
date_updated: 2021-01-03T15:00:59Z
file_id: '652'
file_name: _2020_Wehlage_AIMSbioeng7_43-54.pdf
file_size: 1118818
relation: main_file
success: 1
file_date_updated: 2021-01-03T15:00:59Z
funded_apc: '1'
has_accepted_license: '1'
intvolume: ' 7'
issue: '1'
keyword:
- electrospinning
- nanofiber mat
- autoclaving
- cell growth
- adherent cells
- CHO cells
- DMSO
language:
- iso: eng
oa: '1'
page: 43-54
publication: AIMS Bioengineering
publication_identifier:
issn:
- 2375-1495
publication_status: published
quality_controlled: '1'
status: public
title: Cell growth on electrospun nanofiber mats from polyacrylonitrile (PAN) blends
type: journal_article
user_id: '223776'
volume: 7
year: '2020'
...
---
_id: '166'
abstract:
- lang: eng
text: "Nanofi brous mats can be used as a substrate for eukaryotic cell growth in
biotechnology, tissue engineering, etc. Several adherent cells (e.g. human fibroblasts)
have been shown to grow well on fine fibres. For most applications, it is necessary
to sterilize nanofibrous mats before adding the cells. Another possibility would
be the addition of antibiotics and antimycotics to the cell culture medium to
prevent microbial infection. However, antibiotics are disadvantageous since they
might promote the growth of resistant bacteria in possible future medical applications
of nanofibrous mats. Possible sterilization techniques include autoclaving, UV-sterilization,
ozone treatment, heat sterilization and other techniques which usually necessitate
more expensive equipment, such as gamma irradiation. Systematic examinations of
the infl uence of different sterilization techniques on the cell growth on nanofibrous
mats have not yet been reported in the literature. Here, we report on the first
experimental investigations of the effect of sterilization with different methods
on the properties of polyacrylonitrile (PAN)/gelatine nanofibrous mats, and the
resulting growth\r\nand adhesion of Chinese hamster ovary cells. While all techniques
under investigation yielded sterile nanofibrous mats, autoclaving and heat sterilization
change the PAN/gelatine fibre morphology. Ozone, on the other hand, modifies the
pH value of the culture medium and partly impedes cell adhesion. UV sterilization
also suggests a chemical modification of the nanofibrous mat. Unexpectedly, heat
sterilization resulted in the highest amount of adherent Chinese hamster ovary
cells grown on PAN/gelatine nanofibrous mats in spite of gelatine melting."
article_type: original
author:
- first_name: Daria
full_name: Wehlage, Daria
last_name: Wehlage
- first_name: Hannah
full_name: Blattner, Hannah
last_name: Blattner
- first_name: Lilia
full_name: Sabantina, Lilia
last_name: Sabantina
- first_name: Robin
full_name: Böttjer, Robin
last_name: Böttjer
- first_name: Timo
full_name: Grothe, Timo
id: '221330'
last_name: Grothe
orcid: 0000-0002-9099-4277
orcid_put_code_url: https://api.orcid.org/v2.0/0000-0002-9099-4277/work/94763802
- first_name: Anke
full_name: Rattenholl, Anke
last_name: Rattenholl
- first_name: Frank
full_name: Gudermann, Frank
last_name: Gudermann
- first_name: Dirk
full_name: Lütkemeyer, Dirk
last_name: Lütkemeyer
- first_name: Andrea
full_name: Ehrmann, Andrea
id: '223776'
last_name: Ehrmann
orcid: 0000-0003-0695-3905
orcid_put_code_url: https://api.orcid.org/v2.0/0000-0003-0695-3905/work/94763803
citation:
alphadin: 'Wehlage,
Daria ; Blattner, Hannah
; Sabantina, Lilia ; Böttjer,
Robin ; Grothe, Timo ; Rattenholl, Anke ; Gudermann,
Frank ; Lütkemeyer, Dirk
; u. a.: Sterilization of PAN/Gelatin Nanofibrous Mats for Cell Growth.
In: Tekstilec Bd. 62 (2019), Nr. 2, S. 78–88'
ama: Wehlage D, Blattner H, Sabantina L, et al. Sterilization of PAN/Gelatin Nanofibrous
Mats for Cell Growth. Tekstilec . 2019;62(2):78-88. doi:10.14502/Tekstilec2019.62.78-88
apa: Wehlage, D., Blattner, H., Sabantina, L., Böttjer, R., Grothe, T., Rattenholl,
A., … Ehrmann, A. (2019). Sterilization of PAN/Gelatin Nanofibrous Mats for Cell
Growth. Tekstilec , 62(2), 78–88. https://doi.org/10.14502/Tekstilec2019.62.78-88
bibtex: '@article{Wehlage_Blattner_Sabantina_Böttjer_Grothe_Rattenholl_Gudermann_Lütkemeyer_Ehrmann_2019,
title={Sterilization of PAN/Gelatin Nanofibrous Mats for Cell Growth}, volume={62},
DOI={10.14502/Tekstilec2019.62.78-88},
number={2}, journal={Tekstilec }, author={Wehlage, Daria and Blattner, Hannah
and Sabantina, Lilia and Böttjer, Robin and Grothe, Timo and Rattenholl, Anke
and Gudermann, Frank and Lütkemeyer, Dirk and Ehrmann, Andrea}, year={2019}, pages={78–88}
}'
chicago: 'Wehlage, Daria, Hannah Blattner, Lilia Sabantina, Robin Böttjer, Timo
Grothe, Anke Rattenholl, Frank Gudermann, Dirk Lütkemeyer, and Andrea Ehrmann.
“Sterilization of PAN/Gelatin Nanofibrous Mats for Cell Growth.” Tekstilec
62, no. 2 (2019): 78–88. https://doi.org/10.14502/Tekstilec2019.62.78-88.'
ieee: D. Wehlage et al., “Sterilization of PAN/Gelatin Nanofibrous Mats for
Cell Growth,” Tekstilec , vol. 62, no. 2, pp. 78–88, 2019.
mla: Wehlage, Daria, et al. “Sterilization of PAN/Gelatin Nanofibrous Mats for Cell
Growth.” Tekstilec , vol. 62, no. 2, 2019, pp. 78–88, doi:10.14502/Tekstilec2019.62.78-88.
short: D. Wehlage, H. Blattner, L. Sabantina, R. Böttjer, T. Grothe, A. Rattenholl,
F. Gudermann, D. Lütkemeyer, A. Ehrmann, Tekstilec 62 (2019) 78–88.
date_created: 2019-04-17T11:51:01Z
date_updated: 2024-03-27T14:01:13Z
doi: 10.14502/Tekstilec2019.62.78-88
file:
- access_level: open_access
content_type: application/pdf
creator: mstock1
date_created: 2019-05-28T06:52:24Z
date_updated: 2019-05-28T06:52:24Z
file_id: '274'
file_name: _2019_Wehlage_Tekstilec62_78-88.pdf
file_size: 959760
relation: main_file
success: 1
file_date_updated: 2019-05-28T06:52:24Z
has_accepted_license: '1'
intvolume: ' 62'
issue: '2'
keyword:
- polyacrylonitrile/gelatine nanofibrous mats
- sterilization
- autoclaving
- ozone
- UV sterilization
- heat sterilization
- cell growth
- adherent cells
- CHO cells
- tissue engineering
language:
- iso: eng
oa: '1'
page: 78-88
publication: 'Tekstilec '
quality_controlled: '1'
status: public
title: Sterilization of PAN/Gelatin Nanofibrous Mats for Cell Growth
type: journal_article
urn: urn:nbn:de:hbz:bi10-1661
user_id: '237837'
volume: 62
year: '2019'
...