---
_id: '166'
abstract:
- lang: eng
text: "Nanofi brous mats can be used as a substrate for eukaryotic cell growth in
biotechnology, tissue engineering, etc. Several adherent cells (e.g. human fibroblasts)
have been shown to grow well on fine fibres. For most applications, it is necessary
to sterilize nanofibrous mats before adding the cells. Another possibility would
be the addition of antibiotics and antimycotics to the cell culture medium to
prevent microbial infection. However, antibiotics are disadvantageous since they
might promote the growth of resistant bacteria in possible future medical applications
of nanofibrous mats. Possible sterilization techniques include autoclaving, UV-sterilization,
ozone treatment, heat sterilization and other techniques which usually necessitate
more expensive equipment, such as gamma irradiation. Systematic examinations of
the infl uence of different sterilization techniques on the cell growth on nanofibrous
mats have not yet been reported in the literature. Here, we report on the first
experimental investigations of the effect of sterilization with different methods
on the properties of polyacrylonitrile (PAN)/gelatine nanofibrous mats, and the
resulting growth\r\nand adhesion of Chinese hamster ovary cells. While all techniques
under investigation yielded sterile nanofibrous mats, autoclaving and heat sterilization
change the PAN/gelatine fibre morphology. Ozone, on the other hand, modifies the
pH value of the culture medium and partly impedes cell adhesion. UV sterilization
also suggests a chemical modification of the nanofibrous mat. Unexpectedly, heat
sterilization resulted in the highest amount of adherent Chinese hamster ovary
cells grown on PAN/gelatine nanofibrous mats in spite of gelatine melting."
article_type: original
author:
- first_name: Daria
full_name: Wehlage, Daria
last_name: Wehlage
- first_name: Hannah
full_name: Blattner, Hannah
last_name: Blattner
- first_name: Lilia
full_name: Sabantina, Lilia
last_name: Sabantina
- first_name: Robin
full_name: Böttjer, Robin
last_name: Böttjer
- first_name: Timo
full_name: Grothe, Timo
id: '221330'
last_name: Grothe
orcid: 0000-0002-9099-4277
orcid_put_code_url: https://api.orcid.org/v2.0/0000-0002-9099-4277/work/94763802
- first_name: Anke
full_name: Rattenholl, Anke
last_name: Rattenholl
- first_name: Frank
full_name: Gudermann, Frank
last_name: Gudermann
- first_name: Dirk
full_name: Lütkemeyer, Dirk
last_name: Lütkemeyer
- first_name: Andrea
full_name: Ehrmann, Andrea
id: '223776'
last_name: Ehrmann
orcid: 0000-0003-0695-3905
orcid_put_code_url: https://api.orcid.org/v2.0/0000-0003-0695-3905/work/94763803
citation:
alphadin: 'Wehlage,
Daria ; Blattner, Hannah
; Sabantina, Lilia ; Böttjer,
Robin ; Grothe, Timo ; Rattenholl, Anke ; Gudermann,
Frank ; Lütkemeyer, Dirk
; u. a.: Sterilization of PAN/Gelatin Nanofibrous Mats for Cell Growth.
In: Tekstilec Bd. 62 (2019), Nr. 2, S. 78–88'
ama: Wehlage D, Blattner H, Sabantina L, et al. Sterilization of PAN/Gelatin Nanofibrous
Mats for Cell Growth. Tekstilec . 2019;62(2):78-88. doi:10.14502/Tekstilec2019.62.78-88
apa: Wehlage, D., Blattner, H., Sabantina, L., Böttjer, R., Grothe, T., Rattenholl,
A., … Ehrmann, A. (2019). Sterilization of PAN/Gelatin Nanofibrous Mats for Cell
Growth. Tekstilec , 62(2), 78–88. https://doi.org/10.14502/Tekstilec2019.62.78-88
bibtex: '@article{Wehlage_Blattner_Sabantina_Böttjer_Grothe_Rattenholl_Gudermann_Lütkemeyer_Ehrmann_2019,
title={Sterilization of PAN/Gelatin Nanofibrous Mats for Cell Growth}, volume={62},
DOI={10.14502/Tekstilec2019.62.78-88},
number={2}, journal={Tekstilec }, author={Wehlage, Daria and Blattner, Hannah
and Sabantina, Lilia and Böttjer, Robin and Grothe, Timo and Rattenholl, Anke
and Gudermann, Frank and Lütkemeyer, Dirk and Ehrmann, Andrea}, year={2019}, pages={78–88}
}'
chicago: 'Wehlage, Daria, Hannah Blattner, Lilia Sabantina, Robin Böttjer, Timo
Grothe, Anke Rattenholl, Frank Gudermann, Dirk Lütkemeyer, and Andrea Ehrmann.
“Sterilization of PAN/Gelatin Nanofibrous Mats for Cell Growth.” Tekstilec
62, no. 2 (2019): 78–88. https://doi.org/10.14502/Tekstilec2019.62.78-88.'
ieee: D. Wehlage et al., “Sterilization of PAN/Gelatin Nanofibrous Mats for
Cell Growth,” Tekstilec , vol. 62, no. 2, pp. 78–88, 2019.
mla: Wehlage, Daria, et al. “Sterilization of PAN/Gelatin Nanofibrous Mats for Cell
Growth.” Tekstilec , vol. 62, no. 2, 2019, pp. 78–88, doi:10.14502/Tekstilec2019.62.78-88.
short: D. Wehlage, H. Blattner, L. Sabantina, R. Böttjer, T. Grothe, A. Rattenholl,
F. Gudermann, D. Lütkemeyer, A. Ehrmann, Tekstilec 62 (2019) 78–88.
date_created: 2019-04-17T11:51:01Z
date_updated: 2024-03-27T14:01:13Z
doi: 10.14502/Tekstilec2019.62.78-88
file:
- access_level: open_access
content_type: application/pdf
creator: mstock1
date_created: 2019-05-28T06:52:24Z
date_updated: 2019-05-28T06:52:24Z
file_id: '274'
file_name: _2019_Wehlage_Tekstilec62_78-88.pdf
file_size: 959760
relation: main_file
success: 1
file_date_updated: 2019-05-28T06:52:24Z
has_accepted_license: '1'
intvolume: ' 62'
issue: '2'
keyword:
- polyacrylonitrile/gelatine nanofibrous mats
- sterilization
- autoclaving
- ozone
- UV sterilization
- heat sterilization
- cell growth
- adherent cells
- CHO cells
- tissue engineering
language:
- iso: eng
oa: '1'
page: 78-88
publication: 'Tekstilec '
quality_controlled: '1'
status: public
title: Sterilization of PAN/Gelatin Nanofibrous Mats for Cell Growth
type: journal_article
urn: urn:nbn:de:hbz:bi10-1661
user_id: '237837'
volume: 62
year: '2019'
...