@article{694, abstract = { Cell growth on three-dimensional objects is of high interest for bioprocess engineering of adherent cells, tissue engineering and other biomedical applications. 3D printing by fusion deposition modeling (FDM) is a cheap possibility to generate tailor-made substrates for cell growth. Nevertheless, not all materials are chemically attractive for cells. Polylactic acid (PLA) and polyethylene terephthalate glycol (PETG) have been reported to be suitable polymers for tissue engineering. Thus, they might also be applicable for cost-effective bioprocessing of adherent cell lines. Here we report on the influence of printing material, surface structure, and sterilization method on Chinese hamster ovary (CHO) cell adhesion on a modified, high temperature resistant PLA, a PETG blend, and unmodified PETG, respectively. Our study revealed that CHO cells grew on all polymers tested without further surface modification. Samples could be efficiently chemically sterilized. Additional acid treatment had no significant effect on cell adhesion. }, author = {Sölmann, Sven and Rattenholl, Anke and Blattner, Hannah and Ehrmann, Guido and Gudermann, Frank and Lütkemeyer, Dirk and Ehrmann, Andrea}, journal = {AIMS Bioengineering}, keywords = {3D printing, FDM printing, sterilization, adherent cells, CHO cells}, number = {1}, pages = {25--35}, title = {{ Mammalian cell adhesion on different 3D printed polymers with varying sterilization methods and acidic treatment}}, volume = {8}, year = {2021}, } @article{166, abstract = {Nanofi brous mats can be used as a substrate for eukaryotic cell growth in biotechnology, tissue engineering, etc. Several adherent cells (e.g. human fibroblasts) have been shown to grow well on fine fibres. For most applications, it is necessary to sterilize nanofibrous mats before adding the cells. Another possibility would be the addition of antibiotics and antimycotics to the cell culture medium to prevent microbial infection. However, antibiotics are disadvantageous since they might promote the growth of resistant bacteria in possible future medical applications of nanofibrous mats. Possible sterilization techniques include autoclaving, UV-sterilization, ozone treatment, heat sterilization and other techniques which usually necessitate more expensive equipment, such as gamma irradiation. Systematic examinations of the infl uence of different sterilization techniques on the cell growth on nanofibrous mats have not yet been reported in the literature. Here, we report on the first experimental investigations of the effect of sterilization with different methods on the properties of polyacrylonitrile (PAN)/gelatine nanofibrous mats, and the resulting growth and adhesion of Chinese hamster ovary cells. While all techniques under investigation yielded sterile nanofibrous mats, autoclaving and heat sterilization change the PAN/gelatine fibre morphology. Ozone, on the other hand, modifies the pH value of the culture medium and partly impedes cell adhesion. UV sterilization also suggests a chemical modification of the nanofibrous mat. Unexpectedly, heat sterilization resulted in the highest amount of adherent Chinese hamster ovary cells grown on PAN/gelatine nanofibrous mats in spite of gelatine melting.}, author = {Wehlage, Daria and Blattner, Hannah and Sabantina, Lilia and Böttjer, Robin and Grothe, Timo and Rattenholl, Anke and Gudermann, Frank and Lütkemeyer, Dirk and Ehrmann, Andrea}, journal = {Tekstilec }, keywords = {polyacrylonitrile/gelatine nanofibrous mats, sterilization, autoclaving, ozone, UV sterilization, heat sterilization, cell growth, adherent cells, CHO cells, tissue engineering}, number = {2}, pages = {78--88}, title = {{Sterilization of PAN/Gelatin Nanofibrous Mats for Cell Growth}}, doi = {10.14502/Tekstilec2019.62.78-88}, volume = {62}, year = {2019}, }