---
_id: '694'
abstract:
- lang: eng
text: ' Cell growth on three-dimensional objects is of high interest for bioprocess
engineering of adherent cells, tissue engineering and other biomedical applications.
3D printing by fusion deposition modeling (FDM) is a cheap possibility to generate
tailor-made substrates for cell growth. Nevertheless, not all materials are chemically
attractive for cells. Polylactic acid (PLA) and polyethylene terephthalate glycol
(PETG) have been reported to be suitable polymers for tissue engineering. Thus,
they might also be applicable for cost-effective bioprocessing of adherent cell
lines. Here we report on the influence of printing material, surface structure,
and sterilization method on Chinese hamster ovary (CHO) cell adhesion on a modified,
high temperature resistant PLA, a PETG blend, and unmodified PETG, respectively.
Our study revealed that CHO cells grew on all polymers tested without further
surface modification. Samples could be efficiently chemically sterilized. Additional
acid treatment had no significant effect on cell adhesion. '
article_type: original
author:
- first_name: Sven
full_name: Sölmann, Sven
last_name: Sölmann
- first_name: Anke
full_name: Rattenholl, Anke
last_name: Rattenholl
- first_name: Hannah
full_name: Blattner, Hannah
last_name: Blattner
- first_name: Guido
full_name: Ehrmann, Guido
last_name: Ehrmann
- first_name: Frank
full_name: Gudermann, Frank
last_name: Gudermann
- first_name: Dirk
full_name: Lütkemeyer, Dirk
last_name: Lütkemeyer
- first_name: Andrea
full_name: Ehrmann, Andrea
id: '223776'
last_name: Ehrmann
orcid: 0000-0003-0695-3905
orcid_put_code_url: https://api.orcid.org/v2.0/0000-0003-0695-3905/work/105572547
citation:
alphadin: 'Sölmann, Sven ; Rattenholl,
Anke ; Blattner, Hannah ;
Ehrmann, Guido ; Gudermann,
Frank ; Lütkemeyer, Dirk
; Ehrmann, Andrea: Mammalian cell
adhesion on different 3D printed polymers with varying sterilization methods and
acidic treatment. In: AIMS Bioengineering Bd. 8 (2021), Nr. 1, S. 25–35'
ama: Sölmann S, Rattenholl A, Blattner H, et al. Mammalian cell adhesion on different
3D printed polymers with varying sterilization methods and acidic treatment. AIMS
Bioengineering. 2021;8(1):25-35.
apa: Sölmann, S., Rattenholl, A., Blattner, H., Ehrmann, G., Gudermann, F., Lütkemeyer,
D., & Ehrmann, A. (2021). Mammalian cell adhesion on different 3D printed
polymers with varying sterilization methods and acidic treatment. AIMS Bioengineering,
8(1), 25–35.
bibtex: '@article{Sölmann_Rattenholl_Blattner_Ehrmann_Gudermann_Lütkemeyer_Ehrmann_2021,
title={ Mammalian cell adhesion on different 3D printed polymers with varying
sterilization methods and acidic treatment}, volume={8}, number={1}, journal={AIMS
Bioengineering}, author={Sölmann, Sven and Rattenholl, Anke and Blattner, Hannah
and Ehrmann, Guido and Gudermann, Frank and Lütkemeyer, Dirk and Ehrmann, Andrea},
year={2021}, pages={25–35} }'
chicago: 'Sölmann, Sven, Anke Rattenholl, Hannah Blattner, Guido Ehrmann, Frank
Gudermann, Dirk Lütkemeyer, and Andrea Ehrmann. “ Mammalian Cell Adhesion on Different
3D Printed Polymers with Varying Sterilization Methods and Acidic Treatment.”
AIMS Bioengineering 8, no. 1 (2021): 25–35.'
ieee: S. Sölmann et al., “ Mammalian cell adhesion on different 3D printed
polymers with varying sterilization methods and acidic treatment,” AIMS Bioengineering,
vol. 8, no. 1, pp. 25–35, 2021.
mla: Sölmann, Sven, et al. “ Mammalian Cell Adhesion on Different 3D Printed Polymers
with Varying Sterilization Methods and Acidic Treatment.” AIMS Bioengineering,
vol. 8, no. 1, 2021, pp. 25–35.
short: S. Sölmann, A. Rattenholl, H. Blattner, G. Ehrmann, F. Gudermann, D. Lütkemeyer,
A. Ehrmann, AIMS Bioengineering 8 (2021) 25–35.
date_created: 2021-01-03T18:14:05Z
date_updated: 2022-01-01T15:23:09Z
department:
- _id: '103'
file:
- access_level: open_access
content_type: application/pdf
creator: aehrmann
date_created: 2021-01-03T18:13:31Z
date_updated: 2021-01-03T18:13:31Z
file_id: '695'
file_name: _2021_Sölmann_AIMSBioneng8_25-35.pdf
file_size: 1154128
relation: main_file
success: 1
file_date_updated: 2021-01-03T18:13:31Z
funded_apc: '1'
has_accepted_license: '1'
intvolume: ' 8'
issue: '1'
keyword:
- 3D printing
- FDM printing
- sterilization
- adherent cells
- CHO cells
language:
- iso: eng
main_file_link:
- open_access: '1'
oa: '1'
page: 25-35
publication: AIMS Bioengineering
publication_status: published
quality_controlled: '1'
status: public
title: ' Mammalian cell adhesion on different 3D printed polymers with varying sterilization
methods and acidic treatment'
type: journal_article
user_id: '223776'
volume: 8
year: '2021'
...
---
_id: '166'
abstract:
- lang: eng
text: "Nanofi brous mats can be used as a substrate for eukaryotic cell growth in
biotechnology, tissue engineering, etc. Several adherent cells (e.g. human fibroblasts)
have been shown to grow well on fine fibres. For most applications, it is necessary
to sterilize nanofibrous mats before adding the cells. Another possibility would
be the addition of antibiotics and antimycotics to the cell culture medium to
prevent microbial infection. However, antibiotics are disadvantageous since they
might promote the growth of resistant bacteria in possible future medical applications
of nanofibrous mats. Possible sterilization techniques include autoclaving, UV-sterilization,
ozone treatment, heat sterilization and other techniques which usually necessitate
more expensive equipment, such as gamma irradiation. Systematic examinations of
the infl uence of different sterilization techniques on the cell growth on nanofibrous
mats have not yet been reported in the literature. Here, we report on the first
experimental investigations of the effect of sterilization with different methods
on the properties of polyacrylonitrile (PAN)/gelatine nanofibrous mats, and the
resulting growth\r\nand adhesion of Chinese hamster ovary cells. While all techniques
under investigation yielded sterile nanofibrous mats, autoclaving and heat sterilization
change the PAN/gelatine fibre morphology. Ozone, on the other hand, modifies the
pH value of the culture medium and partly impedes cell adhesion. UV sterilization
also suggests a chemical modification of the nanofibrous mat. Unexpectedly, heat
sterilization resulted in the highest amount of adherent Chinese hamster ovary
cells grown on PAN/gelatine nanofibrous mats in spite of gelatine melting."
article_type: original
author:
- first_name: Daria
full_name: Wehlage, Daria
last_name: Wehlage
- first_name: Hannah
full_name: Blattner, Hannah
last_name: Blattner
- first_name: Lilia
full_name: Sabantina, Lilia
last_name: Sabantina
- first_name: Robin
full_name: Böttjer, Robin
last_name: Böttjer
- first_name: Timo
full_name: Grothe, Timo
id: '221330'
last_name: Grothe
orcid: 0000-0002-9099-4277
orcid_put_code_url: https://api.orcid.org/v2.0/0000-0002-9099-4277/work/94763802
- first_name: Anke
full_name: Rattenholl, Anke
last_name: Rattenholl
- first_name: Frank
full_name: Gudermann, Frank
last_name: Gudermann
- first_name: Dirk
full_name: Lütkemeyer, Dirk
last_name: Lütkemeyer
- first_name: Andrea
full_name: Ehrmann, Andrea
id: '223776'
last_name: Ehrmann
orcid: 0000-0003-0695-3905
orcid_put_code_url: https://api.orcid.org/v2.0/0000-0003-0695-3905/work/94763803
citation:
alphadin: 'Wehlage,
Daria ; Blattner, Hannah
; Sabantina, Lilia ; Böttjer,
Robin ; Grothe, Timo ; Rattenholl, Anke ; Gudermann,
Frank ; Lütkemeyer, Dirk
; u. a.: Sterilization of PAN/Gelatin Nanofibrous Mats for Cell Growth.
In: Tekstilec Bd. 62 (2019), Nr. 2, S. 78–88'
ama: Wehlage D, Blattner H, Sabantina L, et al. Sterilization of PAN/Gelatin Nanofibrous
Mats for Cell Growth. Tekstilec . 2019;62(2):78-88. doi:10.14502/Tekstilec2019.62.78-88
apa: Wehlage, D., Blattner, H., Sabantina, L., Böttjer, R., Grothe, T., Rattenholl,
A., … Ehrmann, A. (2019). Sterilization of PAN/Gelatin Nanofibrous Mats for Cell
Growth. Tekstilec , 62(2), 78–88. https://doi.org/10.14502/Tekstilec2019.62.78-88
bibtex: '@article{Wehlage_Blattner_Sabantina_Böttjer_Grothe_Rattenholl_Gudermann_Lütkemeyer_Ehrmann_2019,
title={Sterilization of PAN/Gelatin Nanofibrous Mats for Cell Growth}, volume={62},
DOI={10.14502/Tekstilec2019.62.78-88},
number={2}, journal={Tekstilec }, author={Wehlage, Daria and Blattner, Hannah
and Sabantina, Lilia and Böttjer, Robin and Grothe, Timo and Rattenholl, Anke
and Gudermann, Frank and Lütkemeyer, Dirk and Ehrmann, Andrea}, year={2019}, pages={78–88}
}'
chicago: 'Wehlage, Daria, Hannah Blattner, Lilia Sabantina, Robin Böttjer, Timo
Grothe, Anke Rattenholl, Frank Gudermann, Dirk Lütkemeyer, and Andrea Ehrmann.
“Sterilization of PAN/Gelatin Nanofibrous Mats for Cell Growth.” Tekstilec
62, no. 2 (2019): 78–88. https://doi.org/10.14502/Tekstilec2019.62.78-88.'
ieee: D. Wehlage et al., “Sterilization of PAN/Gelatin Nanofibrous Mats for
Cell Growth,” Tekstilec , vol. 62, no. 2, pp. 78–88, 2019.
mla: Wehlage, Daria, et al. “Sterilization of PAN/Gelatin Nanofibrous Mats for Cell
Growth.” Tekstilec , vol. 62, no. 2, 2019, pp. 78–88, doi:10.14502/Tekstilec2019.62.78-88.
short: D. Wehlage, H. Blattner, L. Sabantina, R. Böttjer, T. Grothe, A. Rattenholl,
F. Gudermann, D. Lütkemeyer, A. Ehrmann, Tekstilec 62 (2019) 78–88.
date_created: 2019-04-17T11:51:01Z
date_updated: 2024-03-27T14:01:13Z
doi: 10.14502/Tekstilec2019.62.78-88
file:
- access_level: open_access
content_type: application/pdf
creator: mstock1
date_created: 2019-05-28T06:52:24Z
date_updated: 2019-05-28T06:52:24Z
file_id: '274'
file_name: _2019_Wehlage_Tekstilec62_78-88.pdf
file_size: 959760
relation: main_file
success: 1
file_date_updated: 2019-05-28T06:52:24Z
has_accepted_license: '1'
intvolume: ' 62'
issue: '2'
keyword:
- polyacrylonitrile/gelatine nanofibrous mats
- sterilization
- autoclaving
- ozone
- UV sterilization
- heat sterilization
- cell growth
- adherent cells
- CHO cells
- tissue engineering
language:
- iso: eng
oa: '1'
page: 78-88
publication: 'Tekstilec '
quality_controlled: '1'
status: public
title: Sterilization of PAN/Gelatin Nanofibrous Mats for Cell Growth
type: journal_article
urn: urn:nbn:de:hbz:bi10-1661
user_id: '237837'
volume: 62
year: '2019'
...