@article{1732, abstract = {Despite being a promising feedstock for food, feed, chemicals, and biofuels, microalgal production processes are still uneconomical due to slow growth rates, costly media, problematic downstreaming processes, and rather low cell densities. Immobilization via entrapment constitutes a promising tool to overcome these drawbacks of microalgal production and enables continuous processes with protection against shear forces and contaminations. In contrast to biopolymer gels, inorganic silica hydrogels are highly transparent and chemically, mechanically, thermally, and biologically stable. Since the first report on entrapment of living cells in silica hydrogels in 1989, efforts were made to increase the biocompatibility by omitting organic solvents during hydrolysis, removing toxic by-products, and replacing detrimental mineral acids or bases for pH adjustment. Furthermore, methods were developed to decrease the stiffness in order to enable proliferation of entrapped cells. This review aims to provide an overview of studied entrapment methods in silica hydrogels, specifically for rather sensitive microalgae. }, author = {Homburg, Sarah Vanessa and Patel, Anant}, issn = {2073-4360}, journal = {Polymers}, number = {7}, publisher = {MDPI AG}, title = {{Silica Hydrogels as Entrapment Material for Microalgae}}, doi = {10.3390/polym14071391}, volume = {14}, year = {2022}, } @article{2015, abstract = { While fused deposition modeling (FDM) and other relatively inexpensive 3D printing methods are nowadays used in many applications, the possible areas of using FDM-printed objects are still limited due to mechanical and thermal constraints. Applications for space, e.g., for microsatellites, are restricted by the usually insufficient heat resistance of the typical FDM printing materials. Printing high-temperature polymers, on the other hand, necessitates special FDM printers, which are not always available. Here, we show investigations of common polymers, processible on low-cost FDM printers, under elevated temperatures of up to 160 °C for single treatments. The polymers with the highest dimensional stability and mechanical properties after different temperature treatments were periodically heat-treated between -40 °C and +80 °C in cycles of 90 min, similar to the temperature cycles a microsatellite in the low Earth orbit (LEO) experiences. While none of the materials under investigation fully maintains its dimensions and mechanical properties, filled poly(lactic acid) (PLA) filaments were found most suitable for applications under these thermal conditions. }, author = {Storck, Jan Lukas and Ehrmann, Guido and Güth, Uwe and Uthoff, Jana and Homburg, Sarah Vanessa and Blachowicz, Tomasz and Ehrmann, Andrea}, issn = {2073-4360}, journal = {Polymers}, keywords = {additive manufacturing, polymers, space, microsatellites, thermal stability, dimensions, mechanical properties}, number = {14}, publisher = {MDPI AG}, title = {{Investigation of Low-Cost FDM-Printed Polymers for Elevated-Temperature Applications}}, doi = {10.3390/polym14142826}, volume = {14}, year = {2022}, } @article{2031, abstract = { Atomic force microscopy (AFM) is one of the microscopic techniques with the highest lateral resolution. It can usually be applied in air or even in liquids, enabling the investigation of a broader range of samples than scanning electron microscopy (SEM), which is mostly performed in vacuum. Since it works by following the sample surface based on the force between the scanning tip and the sample, interactions have to be taken into account, making the AFM of irregular samples complicated, but on the other hand it allows measurements of more physical parameters than pure topography. This is especially important for biopolymers and hydrogels used in tissue engineering and other biotechnological applications, where elastic properties, surface charges and other parameters influence mammalian cell adhesion and growth as well as many other effects. This review gives an overview of AFM modes relevant for the investigations of biopolymers and hydrogels and shows several examples of recent applications, focusing on the polysaccharides chitosan, alginate, carrageenan and different hydrogels, but depicting also a broader spectrum of materials on which different AFM measurements are reported in the literature. }, author = {Joshi, Jnanada Shrikant and Homburg, Sarah Vanessa and Ehrmann, Andrea}, issn = {2073-4360}, journal = {Polymers}, keywords = {nanoindentation, elastic modulus, peak force quantitative nanomechanical mapping, KPFM, interaction forces, adhesion, impedance, adsorption, ultracentrifugation, drop deposition}, number = {6}, publisher = {MDPI AG}, title = {{Atomic Force Microscopy (AFM) on Biopolymers and Hydrogels for Biotechnological Applications—Possibilities and Limits}}, doi = {10.3390/polym14061267}, volume = {14}, year = {2022}, } @article{2035, abstract = { Microalgae can be used for diverse applications in research and industry. Several microalgae grow adhering to surfaces that are usually two-dimensional. A third dimension could increase the amount of microalgae adhering to a given area and can be offered by textile fabrics. Here we report on the microalgae Chlorella vulgaris and Scenedesmus spec. growing on different knitted fabrics under defined light and under office light conditions. Our results show a significant influence of illumination on both algal species and a smaller impact of the chosen medium, while all knitted fabrics under examination were found well suited as substrates. The numbers of alga cells per petri dish were higher on textile fabrics than in pure water or medium by a factor of ~ 4–20, respectively. }, author = {Tanzli, Ewin and Brockhagen, Bennet and Post, Inken Blanka and Bache, Thorsten and Tuvshinbayar, Khorolsuren and Homburg, Sarah Vanessa and Ehrmann, Andrea}, issn = {2701-939X}, journal = {Communications in Development and Assembling of Textile Products}, keywords = {green microalgae, knitted fabrics, Tencel, cotton, linen, oxygen production, Clark electrode}, number = {1}, pages = {9--16}, publisher = {Sachsische Landesbibliothek, Staats- und Universitatsbibliothek Dresden}, title = {{Microalgae growth and oxygen production on different textile fabrics}}, doi = {10.25367/cdatp.2022.3.p9-16}, volume = {3}, year = {2022}, } @article{2037, abstract = { To measure biosignals constantly, using textile-integrated or even textile-based electrodes and miniaturized electronics, is ideal to provide maximum comfort for patients or athletes during monitoring. While in former times, this was usually solved by integrating specialized electronics into garments, either connected to a handheld computer or including a wireless data transfer option, nowadays increasingly smaller single circuit boards are available, e.g., single-board computers such as Raspberry Pi or microcontrollers such as Arduino, in various shapes and dimensions. This review gives an overview of studies found in the recent scientific literature, reporting measurements of biosignals such as ECG, EMG, sweat and other health-related parameters by single circuit boards, showing new possibilities offered by Arduino, Raspberry Pi etc. in the mobile long-term acquisition of biosignals. The review concentrates on the electronics, not on textile electrodes about which several review papers are available. }, author = {Ehrmann, Guido and Blachowicz, Tomasz and Homburg, Sarah Vanessa and Ehrmann, Andrea}, issn = {2306-5354}, journal = {Bioengineering}, number = {2}, publisher = {MDPI AG}, title = {{Measuring Biosignals with Single Circuit Boards}}, doi = {10.3390/bioengineering9020084}, volume = {9}, year = {2022}, } @inproceedings{3165, author = {Fladung, Laura and Homburg, Sarah Vanessa and Kruse, Olaf and Patel, Anant}, booktitle = {Chemie Ingenieur Technik}, location = {Aachen}, number = {9}, pages = {1257--1257}, publisher = {Wiley}, title = {{Development of novel silica hydrogels with improved structure properties to support growth of entrapped microalgae}}, doi = {10.1002/cite.202255160}, volume = {94}, year = {2022}, } @inproceedings{3164, author = {Fladung, Laura and Homburg, Sarah Vanessa and Kruse, Olaf and Patel, Anant}, booktitle = {Chemie Ingenieur Technik}, location = {Aachen}, number = {9}, pages = {1275--1275}, publisher = {Wiley}, title = {{A novel method to measure diffusion of dissolved CO2 in hydrogels}}, doi = {DOI: 10.1002/cite.202255158}, volume = {94}, year = {2022}, } @inproceedings{3155, author = {Fladung, Laura and Homburg, Sarah Vanessa and Kruse, Olaf and Patel, Anant}, location = { Frankfurt am Main }, title = {{Development of novel silica hydrogels for the encapsulation of photosynthetic microalgae}}, year = {2022}, } @inproceedings{2871, author = {Joshi, Jnanada Shrikant and Homburg, Sarah Vanessa and Kruse, Olaf and Patel, Anant}, location = {Frankfurt am Main}, title = {{Robust microalgal production processes in co-cultivation with immobilized plant growth promoting bacteria}}, year = {2022}, } @inproceedings{2864, author = {Joshi, Jnanada Shrikant and Homburg, Sarah Vanessa and Kruse, Olaf and Patel, Anant}, booktitle = {Chemie Ingenieur Technik}, issn = {1522-2640}, location = {Aachen}, number = {9}, pages = {1275--1275}, publisher = {Wiley}, title = {{High‐resolution microscopy techniques for characterization of immobilized bacteria}}, doi = {10.1002/cite.202255133}, volume = {94}, year = {2022}, } @inproceedings{2862, author = {Joshi, Jnanada Shrikant and Homburg, Sarah Vanessa and Kruse, Olaf and Patel, Anant}, booktitle = {Chemie Ingenieur Technik}, issn = {1522-2640}, number = {9}, pages = {1251--1251}, publisher = {Wiley}, title = {{Co‐cultivation of immobilized plant growth promoting bacteria for robust microalgal production processes}}, doi = {10.1002/cite.202255131}, volume = {94}, year = {2022}, } @inproceedings{2867, author = {Joshi, Jnanada Shrikant and Fladung, Laura and Homburg, Sarah Vanessa and Kruse, Olaf and Patel, Anant}, location = {online}, title = {{Novel application of plant growth promoting bacteria: synergistic co-cultivation with microalgae}}, year = {2021}, } @inproceedings{2866, author = {Joshi, Jnanada Shrikant and Homburg, Sarah Vanessa and Patel, Anant and Kruse, Olaf}, booktitle = {Chemie Ingenieur Technik}, issn = {1522-2640}, location = {online}, number = {9}, pages = {1234--1234}, publisher = {Wiley}, title = {{Immobilization of plant growth promoting bacteria in different polymers for robust microalgae production processes}}, doi = {10.1002/cite.202055348}, volume = {92}, year = {2020}, } @inproceedings{2868, author = {Joshi, Jnanada Shrikant and Homburg, Sarah Vanessa and Kruse, Olaf and Patel, Anant}, location = {Strasbourg, France}, title = {{The project COMBINE: co-cultivation of immobilized microalgae and bacteria}}, year = {2019}, } @article{1736, author = {Homburg, Sarah Vanessa and Kruse, Olaf and Patel, Anant}, issn = {01681656}, journal = {Journal of Biotechnology}, pages = {58--66}, publisher = {Elsevier BV}, title = {{Growth and photosynthetic activity of Chlamydomonas reinhardtii entrapped in lens-shaped silica hydrogels}}, doi = {10.1016/j.jbiotec.2019.06.009}, volume = {302}, year = {2019}, } @inproceedings{543, author = {Storck, Jan Lukas and Homburg, Sarah Vanessa and Feldhans, T. and Bednarz, Hanna and Niehaus, Karsten and Patel, Anant V.}, booktitle = {Special Issue: ProcessNet‐Jahrestagung und 33. DECHEMA‐Jahrestagung der Biotechnologen 2018}, issn = {0009-286X}, number = {9}, pages = {1214--1214}, publisher = {Wiley}, title = {{Microencapsulation as a tool to produce multicellular tumor spheroids}}, doi = {10.1002/cite.201855183}, volume = {90}, year = {2018}, } @article{534, abstract = {In this work, we aimed at improved viability and growth of the microalga *Chlamydomonas reinhardtii* in transparent silica hydrogels based on low-ethanol, low-sodium and low-propylamine synthesis. Investigation into replacement of conventional base KOH by buffers dipotassium phosphate and tris(hydroxymethyl)aminomethane along with increased precursor concentrations yielded an aqueous synthesis route which provided a gelation within 10 min, absorptions below 0.1 and elastic moduli of 0.04-4.23 kPa. The abrasion resistance enhanced by 41 % compared to calcium alginate hydrogels and increased to 70-85 % residual material on addition of chitosan. Entrapment of microalgae in low-sodium and low-propylamine silica hydrogels maintained the PSII quantum yield above 0.3 and growth rates of 0.23 ± 0.01 d-1, similarly to cells entrapped in calcium alginate. These promising results pave the way for the entrapment of sensitive, photosynthetically active and growing cells for in robust biotechnological applications.}, author = {Homburg, Sarah Vanessa and Venkanna, Deepak and Kraushaar, Konstantin and Kruse, Olaf and Kroke, Edwin and Patel, Anant}, issn = {0927-7765}, journal = {Colloids and Surfaces B: Biointerfaces}, pages = {233--241}, publisher = {Elsevier BV}, title = {{Entrapment and growth of Chlamydomonas reinhardtii in biocompatible silica hydrogels}}, doi = {10.1016/j.colsurfb.2018.09.075}, volume = {173}, year = {2018}, } @inproceedings{539, author = {Homburg, Sarah Vanessa and Kruse, Olaf and Patel, Anant}, booktitle = {Special Issue: ProcessNet‐Jahrestagung und 33. DECHEMA‐Jahrestagung der Biotechnologen 2018}, issn = {0009-286X}, number = {9}, pages = {1162--1162}, publisher = {Wiley}, title = {{Viability, growth, and hydrogen production of green microalgae in novel silica hydrogels}}, doi = {10.1002/cite.201855064}, volume = {90}, year = {2018}, } @inproceedings{541, author = {Homburg, Sarah Vanessa and Patel, Anant}, booktitle = {Special Issue: ProcessNet‐Jahrestagung und 33. DECHEMA‐Jahrestagung der Biotechnologen 2018}, issn = {0009-286X}, number = {9}, pages = {1183--1183}, publisher = {Wiley}, title = {{The project COMBINE - Co-cultivation of microalgae and bacteria}}, doi = {10.1002/cite.201855115}, volume = {90}, year = {2018}, } @inproceedings{542, author = {Homburg, Sarah Vanessa and Kruse, Olaf and Patel, Anant}, booktitle = {Special Issue: ProcessNet‐Jahrestagung und 33. DECHEMA‐Jahrestagung der Biotechnologen 2018}, issn = {0009-286X}, number = {9}, pages = {1210--1210}, publisher = {Wiley}, title = {{Development of novel silica hydrogels for entrapment and growth of sensitive microalgae}}, doi = {10.1002/cite.201855173}, volume = {90}, year = {2018}, }