Atomic Force Microscopy (AFM) on Biopolymers and Hydrogels for Biotechnological Applications—Possibilities and Limits

J. Joshi, S.V. Homburg, A. Ehrmann, Polymers 14 (2022).

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Abstract
Atomic force microscopy (AFM) is one of the microscopic techniques with the highest lateral resolution. It can usually be applied in air or even in liquids, enabling the investigation of a broader range of samples than scanning electron microscopy (SEM), which is mostly performed in vacuum. Since it works by following the sample surface based on the force between the scanning tip and the sample, interactions have to be taken into account, making the AFM of irregular samples complicated, but on the other hand it allows measurements of more physical parameters than pure topography. This is especially important for biopolymers and hydrogels used in tissue engineering and other biotechnological applications, where elastic properties, surface charges and other parameters influence mammalian cell adhesion and growth as well as many other effects. This review gives an overview of AFM modes relevant for the investigations of biopolymers and hydrogels and shows several examples of recent applications, focusing on the polysaccharides chitosan, alginate, carrageenan and different hydrogels, but depicting also a broader spectrum of materials on which different AFM measurements are reported in the literature.
Erscheinungsjahr
Zeitschriftentitel
Polymers
Band
14
Zeitschriftennummer
6
Artikelnummer
1267
eISSN
FH-PUB-ID

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Joshi, Jnanada ; Homburg, Sarah Vanessa ; Ehrmann, Andrea: Atomic Force Microscopy (AFM) on Biopolymers and Hydrogels for Biotechnological Applications—Possibilities and Limits. In: Polymers Bd. 14, MDPI AG (2022), Nr. 6
Joshi J, Homburg SV, Ehrmann A. Atomic Force Microscopy (AFM) on Biopolymers and Hydrogels for Biotechnological Applications—Possibilities and Limits. Polymers. 2022;14(6). doi:10.3390/polym14061267
Joshi, J., Homburg, S. V., & Ehrmann, A. (2022). Atomic Force Microscopy (AFM) on Biopolymers and Hydrogels for Biotechnological Applications—Possibilities and Limits. Polymers, 14(6). https://doi.org/10.3390/polym14061267
@article{Joshi_Homburg_Ehrmann_2022, title={Atomic Force Microscopy (AFM) on Biopolymers and Hydrogels for Biotechnological Applications—Possibilities and Limits}, volume={14}, DOI={10.3390/polym14061267}, number={61267}, journal={Polymers}, publisher={MDPI AG}, author={Joshi, Jnanada and Homburg, Sarah Vanessa and Ehrmann, Andrea}, year={2022} }
Joshi, Jnanada, Sarah Vanessa Homburg, and Andrea Ehrmann. “Atomic Force Microscopy (AFM) on Biopolymers and Hydrogels for Biotechnological Applications—Possibilities and Limits.” Polymers 14, no. 6 (2022). https://doi.org/10.3390/polym14061267.
J. Joshi, S. V. Homburg, and A. Ehrmann, “Atomic Force Microscopy (AFM) on Biopolymers and Hydrogels for Biotechnological Applications—Possibilities and Limits,” Polymers, vol. 14, no. 6, 2022.
Joshi, Jnanada, et al. “Atomic Force Microscopy (AFM) on Biopolymers and Hydrogels for Biotechnological Applications—Possibilities and Limits.” Polymers, vol. 14, no. 6, 1267, MDPI AG, 2022, doi:10.3390/polym14061267.
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2022-07-14T17:48:19Z


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