--- res: bibo_abstract: - Encapsulation of hydrolases in biopolymer-based hydrogels often suffers from low activities and encapsulation efficiencies along with high leaching and unsatisfactory recycling properties. Exemplified for the encapsulation of pig liver esterase the coating of alginate and chitosan beads have been studied by creating various biopolymer hydrogel beads. Enzyme activity and encapsulation efficiency were notably enhanced by chitosan coating of alginate beads while leaching remained nearly unchanged. This was caused by the enzymatic reaction acidifying the matrix, which increased enzyme retention through enhanced electrostatic enzyme-alginate interaction but decreased activity through enzyme deactivation. A practical and ready-to-use method for visualising pH in beads during reaction by co-encapsulation of a conventional pH indicator was also found. Our method proves that pH control inside the beads can only be realised by buffering. The resulting beads provided a specific activity of 0.267 μmol ∙ min-1 ∙ mg-1, effectiveness factor 0.88, encapsulation efficiency of 88%, 5% leaching and good recycling properties. This work will contribute towards better understanding and application of encapsulated hydrolases for enzymatic syntheses.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Jan foaf_name: Pauly, Jan foaf_surname: Pauly - foaf_Person: foaf_givenName: Harald foaf_name: Gröger, Harald foaf_surname: Gröger - foaf_Person: foaf_givenName: Anant V. foaf_name: Patel, Anant foaf_surname: Patel foaf_workInfoHomepage: http://www.librecat.org/personId=201870 orcid: 0000-0003-1771-407X bibo_doi: 10.1016/j.jbiotec.2018.05.017 bibo_volume: 280 dct_date: 2018^xs_gYear dct_isPartOf: - http://id.crossref.org/issn/0168-1656 dct_publisher: Elsevier BV@ dct_title: Design, characterisation and application of alginate-based encapsulated pig liver esterase@ ...